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產(chǎn)品概述
| 名稱 | DLD-1 (人結(jié)直腸腺癌上皮細(xì)胞) (STR鑒定正確) |
| 別稱 | DLD 1; DLD1; CoCL3 |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
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| 推薦傳代比例 | 1:3-1:8 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | DLD-1細(xì)胞是由D·L·Dexter和其同事于1977-1979年分離的兩株結(jié)直腸腺癌細(xì)胞株中的一株。在ATCC和其它地方進(jìn)行的DNA指紋鑒定和染色體組型分析表明DLD-1細(xì)胞與HCT-15細(xì)胞相似，說明這兩者是來自同一個(gè)人的不同克隆。DLD-1細(xì)胞和HCT-15細(xì)胞的遺傳起源可通過DNA指紋鑒定證實(shí)，但染色體組型分析顯示它們?nèi)狈θ旧w標(biāo)記一致改變或數(shù)目上一致改變。DLD-1細(xì)胞的CSAp陰性(CSAp-)，p53抗原表達(dá)呈陽性(p53抗原產(chǎn)生了一個(gè)C→T點(diǎn)突變導(dǎo)致241位的Ser→Phe)。DLD-1細(xì)胞角蛋白免疫過氧化物酶染色陽性，癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表達(dá)呈陽性，癌基因N-myc的表達(dá)未做檢測。DLD-1細(xì)胞表達(dá)腫瘤特異性核基質(zhì)蛋白CC-2、CC-3、CC-4、CC-5和CC-6。1979年提交到ATCC的DLD-1細(xì)胞代數(shù)不明且污染了支原體，其后經(jīng)過12周多種抗生素聯(lián)合培養(yǎng)處理，處理之后每周用Hoechst染色和標(biāo)準(zhǔn)培養(yǎng)法檢測。其后連續(xù)11個(gè)月不加抗生素培養(yǎng)，DLD-1細(xì)胞所有的檢測呈陰性。 |
| 年齡（性別） | 男性；成人 |
| 組織來源 | 結(jié)直腸腺癌上皮細(xì)胞 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 腸癌細(xì)胞 |
| 生物安全等級 | BSL-1 |
| 倍增時(shí)間 | ~24-48 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 抗原表達(dá)情況 | Blood Type O.The cells are weakly positive for keratins and vimentin.The cells are positive for keratin by immunoperoxidase staining.DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T |
| 基因表達(dá)情況 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; colon antigen 3. |
| 保藏機(jī)構(gòu) | ATCC; CCL-221 BCRC; 60132 DSMZ; ACC-278 ECACC; 90102540 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鑒定圖
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參考文獻(xiàn)
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EGFR inhibition augments the therapeutic efficacy of the NAT10 inhibitor Remodelin in Colorectal cancer (2025-02-04)
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-025-03277-y
影響因子 ：11.4
引用產(chǎn)品： Caco-2 細(xì)胞 , SW48 細(xì)胞 , DLD-1 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , SW620 細(xì)胞 , HCT 116 細(xì)胞 , DMEM高糖完全培養(yǎng)基（含10% FBS） , MEM 培養(yǎng)基
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Investigating the role of MicroRNA-519d-3p in enhancing chemosensitivity of colorectal cancer cells to 5-Fluorouracil through PFKFB3 targeting (2025-02-26)
期刊：Clinics
DOI：10.1016/j.clinsp.2025.100606
影響因子 ：2.2
引用產(chǎn)品： HT-29 細(xì)胞 , DLD-1 細(xì)胞
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Silencing of SIRPα enhances the antitumor efficacy of CAR-M in solid tumors (2024-10-08)
期刊：Cellular & Molecular Immunology
DOI：10.1038/s41423-024-01220-3
影響因子 ：21.8
引用產(chǎn)品： THP-1 細(xì)胞 , SK-OV-3 細(xì)胞 , DLD-1 細(xì)胞 , AsPC-1 細(xì)胞 , MC38 細(xì)胞 , B16 細(xì)胞 , SK-BR-3 [SKBR3] 細(xì)胞 , ID8 細(xì)胞
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis (2024-01-17)
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2024.01.006
影響因子 ：14.5
引用產(chǎn)品： 特級胎牛血清 , LoVo 細(xì)胞 , HT-29 細(xì)胞 , HCT 116 細(xì)胞 , SW620 細(xì)胞 , RKO 細(xì)胞 , DLD-1 細(xì)胞 , HCT-8 [HRT-18] 細(xì)胞
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CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2 (2024-09-27)
期刊：Cell Death & Disease
DOI：10.1038/s41419-024-07057-y
影響因子 ：8.1
引用產(chǎn)品： LoVo 細(xì)胞 , SW620 細(xì)胞 , HCT 116 細(xì)胞 , 293T [HEK-293T] 細(xì)胞 , DLD-1 細(xì)胞 , SW48 細(xì)胞 , NCM460 細(xì)胞
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VWA2 protein molecular mechanism predicts colorectal cancer: Promoting cell invasion and migration by inhibiting NK cell activation (2024-09-06)
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.135394
影響因子 ：7.7
引用產(chǎn)品： DLD-1 細(xì)胞 , HCT 116 細(xì)胞 , HT-29 細(xì)胞 , LoVo 細(xì)胞
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Norcantharidin Sensitizes Colorectal Cancer Cells to Radiotherapy via Reactive Oxygen Species–DRP1-Mediated Mitochondrial Damage (2024-03-14)
期刊：Antioxidants
影響因子 ：7.0
引用產(chǎn)品： DLD-1 細(xì)胞
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DDR1 is identified as an immunotherapy target for microsatellite stable colon cancer by CRISPR screening (2024-11-07)
期刊：npj Precision Oncology
DOI：10.1038/s41698-024-00743-2
影響因子 ：6.8
引用產(chǎn)品： HT-29 細(xì)胞 , CT26.WT 細(xì)胞 , DLD-1 細(xì)胞 , HCT 116 細(xì)胞
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METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1 (2024-01-24)
期刊：CANCER GENE THERAPY
DOI：10.1038/s41417-024-00731-5
影響因子 ：6.4
引用產(chǎn)品： HCT 116 細(xì)胞 , DLD-1 細(xì)胞 , 293T [HEK-293T] 細(xì)胞
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SERPINB5 promotes colorectal cancer invasion and migration by promoting EMT and angiogenesis via the TNF-α/NF-κB pathway (2024-03-08)
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2024.111759
影響因子 ：5.6
引用產(chǎn)品： DLD-1 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , NCM460 細(xì)胞
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N6-methyladenosine modification of B7-H3 mRNA promotes the development and progression of colorectal cancer (2024-01-18)
期刊：iScience
DOI：10.1016/j.isci.2024.108956
影響因子 ：4.6
引用產(chǎn)品： HCT 116 細(xì)胞 , DLD-1 細(xì)胞
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NAT1 inhibits liver metastasis of colorectal cancer by regulating EMT and glycolysis (2024-06-24)
期刊：Aging-US
影響因子 ：3.9
引用產(chǎn)品： LoVo 細(xì)胞 , DLD-1 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , HCT 116 細(xì)胞
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Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis (2024-01-01)
期刊：Journal of Cancer
影響因子 ：3.9
引用產(chǎn)品： DLD-1 細(xì)胞
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Piperine induces autophagy of colon cancer cells: Dual modulation of AKT/mTOR signaling pathway and ROS production (2024-07-02)
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2024.150340
影響因子 ：2.5
引用產(chǎn)品： HCT 116 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , SW620 細(xì)胞 , DLD-1 細(xì)胞 , CT26.WT 細(xì)胞 , NCM460 細(xì)胞 , IEC-6 細(xì)胞
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WTAP-mediated m6A modification regulates NLRP3/Caspase-1/GSDMD to inhibit pyroptosis and exacerbate colorectal cancer (2024-10-29)
期刊：Biomarkers in Medicine
DOI：10.1080/17520363.2024.2416886
影響因子 ：1.9
引用產(chǎn)品： DLD-1 細(xì)胞 , HCT 116 細(xì)胞 , SW480 [SW-480] 細(xì)胞
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Mollugin suppresses proliferation and drives ferroptosis of colorectal cancer cells through inhibition of insulin-like growth factor 2 mRNA binding protein 3/glutathione peroxidase 4 axis (2023-09-05)
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.115427
影響因子 ：7.5
引用產(chǎn)品： SW620 細(xì)胞 , DLD-1 細(xì)胞
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Identification and validation of a novel prognosis model based on m5C-related long non-coding RNAs in colorectal cancer (2023-09-05)
期刊：Cancer Cell International
DOI：10.1186/s12935-023-03025-2
影響因子 ：5.8
引用產(chǎn)品： NCM460 細(xì)胞 , SW620 細(xì)胞 , HCT 116 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , DLD-1 細(xì)胞 , LoVo 細(xì)胞 , HT-29 細(xì)胞
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Copine 7 promotes colorectal cancer proliferation through PKM2 interaction and MAPK signaling pathway (2023-07-04)
期刊：Frontiers in Oncology
影響因子 ：4.7
引用產(chǎn)品： 293 [HEK-293] 細(xì)胞 , HCT 116 細(xì)胞 , DLD-1 細(xì)胞 , McCoy's 5A 培養(yǎng)基 , 特級胎牛血清 , RPMI-1640 培養(yǎng)基 , DMEM高糖 培養(yǎng)基
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RNF114 facilitates the proliferation, stemness, and metastasis of colorectal cancer (2023-07-27)
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：2.8
引用產(chǎn)品： DLD-1 細(xì)胞 , HT-29 細(xì)胞 , SW480 [SW-480] 細(xì)胞
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA (2022-12-15)
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02551-7
影響因子 ：12.7
引用產(chǎn)品： 293T [HEK-293T] 細(xì)胞 , NCM460 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , DLD-1 細(xì)胞 , HT-29 細(xì)胞 , RKO 細(xì)胞 , HCT 116 細(xì)胞
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Critical role of guanylate binding protein 5 in tumor immune microenvironment and predictive value of immunotherapy response (2022-09-30)
期刊：Frontiers in Genetics
影響因子 ：4.8
引用產(chǎn)品： DLD-1 細(xì)胞
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Lipofectamine 2000? at transfection dose promotes EphA2 transcription in an HDAC4-dependent manner to reduce its cytotoxicity (2022-12-06)
期刊：Heliyon
DOI：10.1016/j.heliyon.2022.e12118
影響因子 ：3.8
引用產(chǎn)品： Hep G2 細(xì)胞 , HuH-7 細(xì)胞 , 293T [HEK-293T] 細(xì)胞 , AGS 細(xì)胞 , DLD-1 細(xì)胞 , Hela 細(xì)胞 , NCI-H460 [H460] 細(xì)胞
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HSPA5 repressed ferroptosis to promote colorectal cancer development by maintaining GPX4 stability (2022-09-01)
期刊：NEOPLASMA
DOI：10.4149/neo_2022_220331N363
影響因子 ：3.4
引用產(chǎn)品： DLD-1 細(xì)胞 , SW480 [SW-480] 細(xì)胞
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CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway (2022-08-15)
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2022.08.036
影響因子 ：3.3
引用產(chǎn)品： LS180 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , HT-29 細(xì)胞 , HCT 116 細(xì)胞 , DLD-1 細(xì)胞
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Circ_KIAA1199 inhibits MSI1 degradation by targeting miR-34c-5p to drive the malignant cell behaviors and tumor growth of colorectal cancer (2022-01-01)
期刊：Anti-Cancer Drugs
DOI：10.1097/CAD.0000000000001164
影響因子 ：2.3
引用產(chǎn)品： HCT 116 細(xì)胞 , DLD-1 細(xì)胞
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Autophagy Induction by Trichodermic Acid Attenuates Endoplasmic Reticulum Stress-Mediated Apoptosis in Colon Cancer Cells (2021-05-25)
期刊：International Journal Of Molecular Sciences
影響因子 ：5.6
引用產(chǎn)品： HCT 116 細(xì)胞 , DLD-1 細(xì)胞
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids (2021-08-15)
期刊：Cancer Biology & Medicine
DOI：10.20892/j.issn.2095-3941.2020.0566
影響因子 ：5.5
引用產(chǎn)品： SW620 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , DLD-1 細(xì)胞 , COLO 205 細(xì)胞
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Cullin-4B promotes cell proliferation and invasion through inactivation of p53 signaling pathway in colorectal cancer (2021-06-08)
期刊：Pathology Research And Practice
影響因子 ：2.8
引用產(chǎn)品： LoVo 細(xì)胞 , SW48 細(xì)胞 , HT-29 細(xì)胞 , DLD-1 細(xì)胞
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The combination of lactoferrin and linolenic acid inhibits colorectal tumor growth through activating AMPK/JNK-related apoptosis pathway (2021-05-31)
期刊：PeerJ
影響因子 ：2.7
引用產(chǎn)品： Hep G2 細(xì)胞 , AGS 細(xì)胞 , HCT 116 細(xì)胞 , DLD-1 細(xì)胞 , HT-29 細(xì)胞
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Ubiquitination-mediated degradation of SIRT1 by SMURF2 suppresses CRC cell proliferation and tumorigenesis (2020-05-02)
期刊：Oncogene
影響因子 ：8.0
引用產(chǎn)品： 293T [HEK-293T] 細(xì)胞 , HCT 116 細(xì)胞 , DLD-1 細(xì)胞
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway (2019-11-03)
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影響因子 ：4.0
引用產(chǎn)品： Caco-2 細(xì)胞 , DLD-1 細(xì)胞 , LoVo 細(xì)胞 , SW480 [SW-480] 細(xì)胞 , T84 細(xì)胞
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